The inflammatory markers, interleukin-1 (IL-1α) and CRP, were also notably decreased after ZnO NPs treatment, concomitant with an increase in nitric oxide (NO) and serum antioxidant enzyme (PON-1) levels in diabetic rats. Zinc oxide nanoparticles (ZnO NPs) are used in an increasing number of industrial products such as rubber, paint, coating, and cosmetics. carried out a detailed study about ZnO NPs against Vibrio cholerae (two biotypes of cholera bacteria (classical and El Tor)). Zinc oxide nanoparticles (ZnO NPs) also have remarkable optical, physical, and antimicrobial properties and therefore have great potential to enhance agriculture. the whole article in a third party publication with the exception of reproduction
The results proved that the occurrence of autophagy in cancer cells was related to intracellular ROS generation. We believe that nanomaterials would dramatically promote the development of medicine, and ZnO nanoparticles are expected to make more exciting contributions in these fields. It easily diffuses into the food material, kill the microbes, and prevent human being from falling ill. The Advancing of Zinc Oxide Nanoparticles for Biomedical Applications, State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China, Department of Chemistry, Jinan University, Guangzhou, China, ZnO NPs suppressed cell viability in Caco-2 cell line via increased ROS and induced IL-8 release [, ZnO NPs and fatty acids could induce lysosomal destabilization in Caco-2 cells [, ZnO NPs induced Caco-2 cells cytotoxicity associated with increased intracellular Zn ions [, ZnO NPs conjugated with peptides had a higher antiproliferation in HT-29 colon cancer cells than other Au NPs and Fe, ZnO NPs caused ROS generation and oxidative DNA damage and lead to mitochondrial-mediated apoptosis in HepG2 cells [, ZnO NPs selectively induce apoptosis in HepG2 cells, which was also mediated by ROS via the p53 pathway [, Dox-ZnO nanocomplex can act as a drug delivery system to increase the internalization of the anticancer drug Dox in SMMC-7721 cells [, Ecofriendly formulated ZnO NPs arrest the cell cycle in the G2/M phase and upregulated proapoptotic genes p53, p21, Bax, and JNK and downregulated antiapoptotic genes Bcl-2, AKT1, and ERK1/2 in a dose-dependent manner in MCF-7 cells [, A doxorubicin delivery system based on zinc oxide nanomaterials can bypass the P-gp increase in the drug accumulation in resistant MCF-7R and MCF-7S cells [, RGD (Arg-Gly-Asp)-targeted ZnO NPs can target integrin, FA-functionalized PTX-ZnO NPs released â¼75% of the paclitaxel payload within six hours in acidic pH, improved chemotherapy tolerance, and increased antitumor efficacy [, ZnO NPs incorporated in liposomes not only rendered pH responsivity to the delivery carrier but also exhibited synergetic chemo-photodynamic anticancer action [, Human lung adenocarcinoma cells with an EGFR mutation are sensitive to ZnO NP20 and Al-ZnO NP20, which resulted in nonautophagic cell death [, ZnO NPs are able to induce significant cytotoxicity, apoptosis, and autophagy in SKOV3 cells through reactive oxygen species generation and oxidative stress [, DOX-ZnO/PEG nanocomposites exhibited better dose-dependent toxicity towards HeLa cell lines [, ZnO nanoparticles showed a dynamic cytotoxic effect in cervical carcinoma cells which induced the apoptosis through the increased intracellular ROS level and upregulated apoptotic gene p53 and caspase-3 expression [, PMMA-AA/ZnO NPs and PMMA-PEG/ZnO were able to carry a large amount of the hydrophobic drug (curcumin) showing highly anti-gastric cancer activity [, ZnO NPs induce cell death at high concentrations, and at lower concentrations, they induce cell cycle arrest in the S and G 2/M phase by intracellular ROS generation in A431 cells [, HA/ZnO nanocomposite caused G2/M cell cycle arrest and stimulated apoptosis-related increase in caspase-3 and â7 activities of the HL-60 cells [, Destroy the membrane integrity and ROS production [, Depolarization of the membrane structure, increased permeabilization and damage of DNA, and generation of ROS [, Alter the bacterial cell membrane permeability and high level of ROS [, Inhibit adenylyl cyclase activity, and cAMP levels are decreased [, Penetrated the cell and caused bacterial cell death [, Attributed to the high affinity of GA for the bacterial cell membrane and the increased lipophilicity upon the addition of GA [, ZnO NPs in combination with thiamine-improved diabetes therapy [, ZnO NPs effectively reversed diabetes-induced pancreatic injury [, ZnO-RSW NPs showed excellent activity against the crude murine pancreatic glucosidase as compared to the individual ZnO NPs and the RSW extract [, ZnO NPs acted as a potent antidiabetic agent evidenced by improved glucose disposal, insulin levels, and zinc status in diabetic rats [, ZnO NPs presented pleiotropic antidiabetic effects via improved insulin signaling, enhanced glucose uptake, decreased hepatic glucose output, decreased lipolysis, and enhanced pancreatic beta cell mass [, ZnO NPs and Ag NPs had more potent antihyperglycemic activity than CeO, Spherical: 96â115ânm; hexagonal: 57â±â0.3ânm, ZnO NPs displayed better antidiabetic potential (IC, ZnO NPs and vildagliptin have synergistic effects on the therapy of type-2 diabetes [, ZnO NPs could improve glucose tolerance and higher serum insulin and reduce blood glucose, nonesterified fatty acids, and triglycerides [, A rapid improvement of AD severity, pruritus, and subjective sleep quality when AD patients wore the ZnO textiles overnight on 3 consecutive days [, ZnO NPs exerted higher anti-inflammatory properties by decreasing drastically on proinflammatory cytokines in the mouse model of AD [, ZnO NPs relieved inflammation and displayed a dose-dependent effect in the suppression of related protein expressions [, ZnO NPs exhibited excellent anti-inflammatory activity with 66.78â, TNTs/ZnO had a significant inhibitory effect on the proliferation and adhesion of macrophages [, ZnO NPs had a higher potential for anti-inflammatory (79%) in comparison with Ag NPs (69.1%) [, The anti-inflammatory abilities of ZnO NPs to suppress proinflammatory cytokines IL-1, Dietary supplementation with ZnO NPs was effective in inhibiting mRNA expression of inflammatory cytokines (IFN-, With tunable photoluminescence emission and high quantum yield, under UV light, ZnO-1 showed green fluorescence, while ZnO-2 appeared yellow [, The PDMAEMA-modified ZnO QDs emitted strong yellow luminescence under UV light [, ZnO-Au@PEG NPs can penetrate into the living cells and exhibit bright yellow fluorescence [, Yellow-orange light emission was observed around or inside the cells under UV light [, Monodispersed ZnO@silica NPs with blue, green, and yellow emission through using VTES, TEOS, and APS as modification materials [, ZnO@silica NPs exhibited emission colors of blue, green, yellow, and orange under 365ânm excitation via the adjustment of the pH of the precipitation solutions [, ZnO@silica QD colloidal solution exhibited a significant blue emission [, ZnO nanowires exhibited green fluorescent. In the future, we believe ZnO NPs can be explored as antibacterial agents, such as ointments, lotions, and mouthwashes. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Cancer, a condition of uncontrolled malignant cell proliferation, is typically treated by chemotherapy, radiotherapy, and surgery in the past several decades. (2008). The particle sizes of zinc oxide nanoparticles were determined by AFM (AFM, SPM-9500J3, Shimadzu CO., Japan) and Zetasizer Nano-ZS90 (Malvern Instruments) by the method of Du et al. Nowadays, the development of green chemistry has attracted more and more attention because it is mostly environmentally friendly [23]. The mitochondrial electron transport chain is known to be associated with intracellular ROS generation, and anticancer agents entering into cancer cells could destroy the electron transport chain and release huge amounts of ROS [58, 59]. HeLa cell spheroids were cultured and the spheroids allowed to form prior to nanoparticle treatment and were exposed to ZnO NP at 10 or 20 ug/ml concentration respectively in serum containing media and photographed at time 0, 24 and 5 days by light ⦠Authors contributing to RSC publications (journal articles, books or book chapters)
49.Jamdagni P, Khatri P, Rana JS. Abstract Biosynthesis of zinc oxide nanoparticles (ZnO-NPs) was achieved by utilizing the reducing and capping potential of leaf, stem and callus aqueous extracts of Mussaenda frondosa. Kitture et al. But the morphology of the ZnO NPs strongly depends on the milling time of the reactant mixture, a longer time of milling led to a smaller particle size. To stabilize ZnO NPs in water, they encapsulated the ZnO NPs with silica to form ZnO@silica core-shell nanostructures. The exact physical and chemical properties of zinc oxide nanoparticles depend on the different ways they are synthesized. Due to inherent toxicity of ZnO NPs, they possess strong inhibition effects against cancerous cell and bacteria, by inducing intracellular ROS generation and activating apoptotic signaling pathway, which makes ZnO NPs a potential candidate as anticancer and antibacterial agents. ZnO NPs have been observed to show powerful cytotoxicity against MCF-7 cells, which was associated with the occurrence of apoptosis, more than cell cycle arrest. It appeared to increase the toxicity of the ZnO NPs to breast cancer MCF-7 and MDA-MB-231 cells at lower doses. A. Othman, C. Greenwood, A. F. Abuelela et al., âCorrelative light-electron microscopy shows RGD-targeted ZnO nanoparticles dissolve in the intracellular environment of triple negative breast cancer cells and cause apoptosis with intratumor heterogeneity,â, N. Puvvada, S. Rajput, B. N. Kumar et al., âNovel ZnO hollow-nanocarriers containing paclitaxel targeting folate-receptors in a malignant pH-microenvironment for effective monitoring and promoting breast tumor regression,â, N. Tripathy, R. Ahmad, H. A. Ko, G. Khang, and Y. Moghaddam et al. Therefore, exposure to them is unavoidable, which makes it ⦠Sharma et al. To assess the effect of nanoparticles (NPs) like nano-zinc oxide (nZnO) and nano-silicon (nSi) on mango tree growth, yield, and fruit quality under salinity conditions, a factorial experiment was conducted using twelve treatments; three replicates each. Hence, autophagy not only promotes cell survival but also activates lethal mechanisms in cancer cells, thus be considered as an important event in nanoparticle-induced cytotoxicity. It also participates in insulin synthesis, storage, and secretion [102]. Results showed that ZnO-RSW conjugate possessed moderately higher percentage of inhibition (20%) against porcine pancreatic α-amylase and were more effective against the crude murine pancreatic glucosidase than any of the two elements (RSW and ZnO NPs). explored the effects of ZnO NPs on human liver cancer HepG2 cells and its possible pharmacological mechanism [42].
ZnO NPs can be selected as an antibacterial material because of its superior properties, such as high specific surface area and high activity to block a wide scope of pathogenic agents. elucidated the efficiency of ZnO nanoparticles (NPs) against different cancer cell lines. The solid was redispersed in 1 mL of water and centrifuged again. Schematic illustration of antibacterial activity of ZnO NPs. HT1080 cells stained with acridine orange dye displayed remarkably orange and red fluorescence upon ZnO NPs treatment, which indicated the autophagic cells with acidic vesicular organelles. So we can believe that eluted Zn2+ from ZnO NPs also take a key role in antibacterial action. They have a large surface area relative to their size and high catalytic activity. Since the advent of nanoparticles and considering these biological activities of zinc ions, the anti-inflammatory effects of ZnO NPs have also attracted much attention. Unlike other metal oxide nanoparticles, such as titanium dioxide, cerium oxide, and iron oxide, ZnO nanoparticles are not highly stable and tend to dissolve in aqueous solutions, subsequently releasing zinc ions from the particles.15â18 The solubility of ZnO nanoparticles depends on pH, concentration, particle size, and the presence of organic compounds.15,19,20 Thus, their instability ⦠Compared with bZnO, nZnO exerted higher anti-inflammatory properties by decreasing drastically on proinflammatory cytokines (IL-10, IL-13, IFN-γ, and Th2 cytokines) in the mouse model of AD. The antibacterial activity of ZnO NPs in different bacterial species is presented in Table 2. Zinc oxide nanoparticle is one such inorganic metal oxide which fulfills all the above requirements, and hence, it can safely be used as medicine, preservative in packaging, and an antimicrobial agent [45, 46]. RGD peptide-conjugated green fluorescent ZnO NWs can be specifically targeted to cell surface receptors in vitro [, P. K. Mishra, H. Mishra, A. Ekielski, S. Talegaonkar, and B. Vaidya, âZinc oxide nanoparticles: a promising nanomaterial for biomedical applications,â, T. G. Smijs and S. Pavel, âTitanium dioxide and zinc oxide nanoparticles in sunscreens: focus on their safety and effectiveness,â, J. It was found that zinc oxide calcined from 400°C to 550°C exhibited the same crystallite growth rate (38â50ânm) [22]. However, excessive ROS will lead to mitochondrial damage and result in the loss of protein activity balance that finally causes cell apoptosis [60]. The antidiabetic activity was assessed with the help of α-amylase and α-glucosidase inhibition assay with murine pancreatic and small intestinal extracts [103]. Furthermore, the luminescence was very stable during cell culturing and the cells were alive at 45âmin of exposure. Rats were injected intraperitoneally with the respected materials, twice/week for eight consecutive weeks. Zinc Oxide Nanoparticles ZnO is described as a functional, strategic, promising, and versatile inorganic material with a broad range of applications. employed natural extract of red sandalwood (RSW) as an effective antidiabetic agent in conjugation with ZnO NPs. Mechanistic studies had proved that the loss of mitochondrial membrane potential-mediated HepG2 cell apoptosis was mainly due to the decrease in mitochondrial membrane potential and Bcl-2/Bax ratios as well as accompanying with the activation of caspase-9. ZnO NPs have exhibited promising biomedical applications based on its anticancer, antibacterial, antidiabetic, anti-inflammatory, drug delivery, as well as bioimaging activity. Bai et al. The ZnO@polymer core-shell nanoparticles exhibited high quantum yield and very stable broad photoluminescence in aqueous solutions. Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by the impairment of the skin-barrier functions, which was involved with complex interaction between genetic and environmental factors [112, 113]. It should be noted that S. aureus was more susceptible to Phβ-GBP-ZnO NPs than P. vulgaris. Some possible ways to produce ZnO nano-particles are laser ablation, hydrothermal methods, electrochemical depositions, solâgel method, chemical vapor deposition, thermal decomposition, 48.Goh EG, Xu X, McCormick PG. We now gradually discover that the impact of zinc on the health of our body might be as far-reaching as that of iron. As shown in Figure 3, within human hepatoma cells, ZnO-1 (derived from LiOH) with an average size of 3ânm showed green fluorescence, while ZnO-2 (derived from NaOH) with an average size of 4ânm appeared yellow. 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